Phylogenetic networks computation made easy. SplitsTree4 is the leading application for computing unrooted phylogenetic networks from molecular sequence data. Given an alignment of sequences, a distance matrix or a set of trees, the program will compute a phylogenetic tree or network using methods such as split decomposition, neighbor-net, consensus network, super networks methods or methods for computing hybridization or simple recombination networks.

Evolutionary relationships are usually represented using phylogenetic trees, based on a model of evolution dominated by mutations and speciation events. More realistic models must also account for gene genesis, loss and duplication events, hybridization, horizontal gene transfer or recombination. Here, phylogenetic networks have a role to play.

Moreover, network methods also provide a value tool for phylogenetic inference even when reticulation events do not play an important role. The combined eect of sampling error and systematic error makes phylogenetics an uncertain science, and network methods provide tools for representing and quantifying this uncertainty.

The aim of SplitsTree4 is to provide a framework for evolutionary analysis using both trees and networks. The program takes as input a set of taxa represented by characters (that is, aligned sequences), distances, quartets, trees or splits and produces as output trees or networks using a number of dierent methods.

Requirements:
* Java

The license of this software is Freeware, you can free download and free use this graphing software.