Mass-spectrometry data analysis made easy. multiplierz provides a suite of tools that can be accessed via the desktop graphical user interface or through custom .mz scripts.

multiplierz is an open-source Python-based environment that provides a scriptable framework for efficient access to manufacturers' proprietary data files via mzAPI. User-defined customization, flexibility, and ease-of-use are central themes in our development efforts. Built-in multiplierz tools include:Automated download of peptide database search results. Built-in multiplierz tools include:
1. Identification driven annotation of precursors
2. Tools to support iTRAQ and label-free quantification
3. Multiple file consolidation and manipulation
4. Interactive viewer for dynamic access to spectra
5. Retrieval of Genbank information
6. In-silico digestion and fragmentation

multiplierz tools are either accessed through the tabs in the main window or through the "Tools" menu. Note: There are two arrows to the right of the tabs that scroll to other tools such as a peptide identification results converter and an interactive console.

multiplierz "Tab" Tools:
1. Download Mascot:
This tool downloads Mascot search results and generates spreadsheet reports. The name of the report is the same as the input peak list or raw file name.

2. Multifile Detect:
The Multifile Detect tool generates a spreadsheet indicating how many of the input files contain a matching entry in a set up specified fields. For example, across how many runs is a common peptide sequence found.

3. Filter Multifiles:
The Filter Multifiles tool runs simple database filter/join operations on multiple input spreadsheets.

4. Download GenBank:
This tool takes as input a multiplierz spreadsheet containing "GI" accession numbers and adds a new sheet containing a variety of GenBank information with hyperlinks to the Entrez Gene webpage.

5. Fragmenter:
The Fragmenter tool generates a table of in-silico fragmented product ions given a peptide sequence and a list of ion types.

6. Digester:
The Digester tool in-silico digests a given set of proteins using a given enzymatic digestion rule and generates a table of peptide digests along with 1+, 2+, and 3+ mz values for each digest.

7. Retrieve Peaks:
The Retrieve Peaks tool accesses the underlying raw MS file to extract precursor and product spectrum information given a set of peptide identifications.

8. Mascot Web Extract:
This tool "screen-scrapes" information such as annotated MS/MS spectrum images, modification position, and protein coverage information from Mascot search result web pages.

9. Formatter:
The Formatter tool converts various search result formats into multiplierz spreadsheets. Supported result formats include Mascot and ProteinPilot.

10. Info File Creator:
This tool generates a helper file for a given raw MS data file that can speed up data access in the future.

11. Console:
The Console allows users to enter script commands for exploring scripting capabilities.

Requirements:
* Python

The license of this software is Free, you can free download and free use this graphing software.