RecodeData
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RecodeData recode genetic marker data with this tool, help you recode genetic marker data in such a way that all alleles are population specific.
This is handy if you want to calculate the standardized genetic differentiation measure that was suggested by Hedrick (2005, Evolution).
RecodeData makes it possible to estimate a standardized measure for other Fst-estimators than Gst (though not Rst), plus that it makes it possible to estimate Gst with correction for sampling bias (Meirmans, 2006).
How do I calculate the standardized measure?
1. Prepare a datafile in Fstat-format, and make sure that is has the extension ".dat".
2. Use Fstat (Goudet, 1995), or another program, to calculate the value of your favourite Fst-estimator (e.g. Weir & Cockerham's (1984) Theta or Nei's (1987) Gst).
3. Use RecodeData to recode the data in the Fstat file, the use of this program should be very straightforward. Make sure the input-file is in the same folder as the program. When you start the program you will be asked for the name of the input-file and for a name for the output-file, and that's it.
4. Use Fstat again to calculate the same Fst-estimator for the recoded data file. This Fstvalue is the maximum value possible given the present within-population variation.
5. The standardized genetic differentiation measure can now be found by dividing the original Fst-value by the maximum value. In other words, the value obtained from the original data-file should be divided by the value obtained from the recoded data-file.
The license of this software is Freeware, you can free download and free use this calculator software.